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Objective To compare the analgesic effect of infiltration anesthesia and femoral nerve block after total knee arthroplasty .Methods Seventy patients with unilateral knee arthroplastywere selected , and they were divided into local anesthesia group (group A) and ultrasound guided femoral nerve block group(group B) by randomized digital method,35cases in each group .All of them were treated with laryngeal mask ventilation under general anesthesia and post-cold therapy.The visual analogue scale (VAS) at postoperative 6h,12h,1d,2d,3d,quadriceps muscle strength at 6h,12h,1d,2d,3d and 5d after operation,range of motion(ROM) at discharge,patients'satisfaction rate, postoperative morphine consumption , incidence of adverse reaction were observed and recorded in the two groups . Results Postoperative 2d,3d,5d,the VAS scores of group A were (3.41 ±0.9)points,(3.19 ±1.24)points,(2.71 ± 0.51)points,respectively,which of group B were (3.35 ±0.8)points,(3.26 ±1.95)points,(2.89 ±0.92)points, respectively,postoperative 2 days the VAS score began to decrease in the two groups (t=2.64,2.52,2.32,all P>0.05),and the pain was alleviated.Postoperative 3d,the femoral quadriceps muscle power of group A was (4.51 ± 0.24)grades,that of group B was (4.12 ±0.63)grades,the,quadriceps muscle strength had improved trend (t=3.82,P>0.05).At discharge from hospital,knee joint range of motion of group A was (92.74 ±15.61) °,which of group B was (90.82 ±12.43) °,the knee joint activity scope of the two groups expanded (t=23.64,P<0.01). Postoperative morphine consumption of group B[(7.4 ±0.8)mg]was lower than group A[(8.7 ±1.2)mg],and the difference between the two groups was statistically significant (t=6.32,P<0.05).Neither group showed any adverse effects of wound infection and respiratory inhibition.There were 8 cases of nausea and vomiting in group A ,9 cases of urinary retention ,10 cases of nausea and vomiting in group B , 10 cases with urinary retention , but there were no statistically significant differences between the two groups (all P>0.05).Conclusion Local infiltration anesthesia and femoral nerve block can achieve good analgesic effect in TKA surgery ,the effect is similar .The latter's satisfaction rate is higher ,and usage amount of morphine is less ,the latter is more suitable for TKA postoperative analgesia .
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After several in -depth investigations on the third party mediation mechanism of "Guangdong mode" in Guangdong Harmony Medical Dispute Mediation Committee and combined with the analysis of the domes-tic and foreign medical dispute disposal experience , this paper summed up five points that successful construction of harmonious mediation mechanism should grasp .The five points include the fairness of harmonious mediation thoughts , the feasibility of harmonious mediation paths , the justice of harmonious mediation mechanism , the suita-bility of harmonious mediation environment and the success of harmonious mediation results .Only when grasp these five points, couldit create a gradually harmonious doctor -patient relationship .
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After several in -depth investigations on the third party mediation mechanism of "Guangdong mode" in Guangdong Harmony Medical Dispute Mediation Committee and combined with the analysis of the domes-tic and foreign medical dispute disposal experience , this paper summed up five points that successful construction of harmonious mediation mechanism should grasp .The five points include the fairness of harmonious mediation thoughts , the feasibility of harmonious mediation paths , the justice of harmonious mediation mechanism , the suita-bility of harmonious mediation environment and the success of harmonious mediation results .Only when grasp these five points, couldit create a gradually harmonious doctor -patient relationship .
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This paper summarizes the connotation and value of humanistic education. According to the characteristics of the ethicality and social ability of medicine and comparing to the international medical humanistic education standard, it analyzes the present situation and difficulties we are facing and puts for-ward some strategies and measures such as renewing humanity educational ideas, building two dimensional curriculum system of General and Professional Course, cultivating and strengthening teachers team with both professional ability and humanity equality and confirming principles of humanistic education. These measures can increase the connotation of humanistic education and extend its extension. They have great practical significance towards cultivating outstanding medical talents with high comprehensive quality and healthy personality.
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Objective To analyze the epidemiologic features of hand-foot-mouth disease (HFMD) in Haikou city from 2008 to 2015.Methods Descriptive methods on epidemiology and detection on pathogens were conducted in Haikou city from 2008 to 2015.Results A total of 71 611 patients were diagnosed as HFMD in Haikou city from 2008 to 2015,including 728 severe cases,accounting for 1.02％ among all the cases.The average annual incidence was 458.89/100 000.A total of 11 deaths were caused by the disease,with the average annual mortality rate as 0.07/100 000.Two peaks of incidence were seen,from April to July and from September to November.Age of the patients mainly fell in children aged 5 and below,taking up 95.78％ of the total cases.Among all the patients,1-year-olds presented the highest incidence as 12 881.24/100 000.The reported incidence for males was higher than that in females.There were 4 districts in Haikou city that reported the disease.Residential areas of the patients were scattered around,with a percentage of 79.89％.Spectrums of pathogens that causing the prevalence of HFMD were EV71 type,Cox A16 type and other enteroviruses,which prevailing in turns,since 2011.Conclusions Haikou city had been an area with high incidence of HFMD.The incidence started to show a rising trend recently.It is suggested that programs as surveillance,case management,health education and comprehensive prevention and control of disease on HFMD targeting on key population should be intensively implemented to reduce the mortality of the disease.
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Objective To analyze the epidemiologic features of hand-foot-mouth disease (HFMD) in Haikou city from 2008 to 2015.Methods Descriptive methods on epidemiology and detection on pathogens were conducted in Haikou city from 2008 to 2015.Results A total of 71 611 patients were diagnosed as HFMD in Haikou city from 2008 to 2015,including 728 severe cases,accounting for 1.02％ among all the cases.The average annual incidence was 458.89/100 000.A total of 11 deaths were caused by the disease,with the average annual mortality rate as 0.07/100 000.Two peaks of incidence were seen,from April to July and from September to November.Age of the patients mainly fell in children aged 5 and below,taking up 95.78％ of the total cases.Among all the patients,1-year-olds presented the highest incidence as 12 881.24/100 000.The reported incidence for males was higher than that in females.There were 4 districts in Haikou city that reported the disease.Residential areas of the patients were scattered around,with a percentage of 79.89％.Spectrums of pathogens that causing the prevalence of HFMD were EV71 type,Cox A16 type and other enteroviruses,which prevailing in turns,since 2011.Conclusions Haikou city had been an area with high incidence of HFMD.The incidence started to show a rising trend recently.It is suggested that programs as surveillance,case management,health education and comprehensive prevention and control of disease on HFMD targeting on key population should be intensively implemented to reduce the mortality of the disease.
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Objective To understand healthcare-associated infection(HAI)in a maternal and child health care hos-pital,so as to provide scientific evidences for further targeted surveillance.Methods A cross-sectional survey was performed by bedside visiting and medical record reviewing.Results Of 768 hospitalized patients,9(1 .18%)had HAI,the top 3 highest prevalence rates were found in obstetrical intensive care unit (9.09%),neonatal intensive care unit (5.80%)and gynecological department II(2.22%).Antimicrobial usage rate was 30.34%(n=233),134 of which (57.51 %)were prophylactic use,165 were mono-therapy(70.82%).A total of 5 pathogenic bacteria were isolated,the number of Streptococcus agalactiae ,Klebsiella pneumonia ,Enterococcus faecalis ,and Staphylococcus saprophyticus was 2,1 ,1 ,and 1 respectively,except Streptococcus agalactiae ,the other 3 strains were multidrug-resistant organisms(MDROs).Conclusion Surveillance on MDRO infection should be paid much attention,the oc-currence of MDRO infection should be reduced through targeted and bundle intervention.
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Procalcitonin ( PCT) has closely related to the severity of senile pulmonary infection, and its dynamic detection can be used as the auxiliary diagnosis index for elderly severe pneumonia ( SP) . The effects of serum PCT dynamic detection in the early quick identification, diagnosis, disease course monitoring and prognosis evaluation for elderly SP were reviewed, and the effects and clinical significance of the detection in the optimization of antibiotic therapy were also summarized in the paper. The results showed the impor-tant value of PCT dynamic detection in the diagnosis of elderly SP and anti-infection therapy.
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<p><b>BACKGROUND</b>Found in inflammatory zone 1 (FIZZ1) protein increased in pulmonary epithelial cells and in limited amounts of other lung cells. FIZZ1 increased in murine model of smoke induced chronic obstructive pulmonary disease. However, the direct role of FIZZ1 produced by pulmonary epithelium stimulated with cigarette smoke extraction has not been determined. We examined the expression and function of FIZZ1 in rat lung epithelial L2 cells.</p><p><b>METHODS</b>The rat lung epithelial L2 cells (CCL 149) were exposed to cigarette smoke extraction, expression of FIZZ1 mRNA was investigated by RT-PCR. Levels of FIZZ1 protein were detected by Western blotting and laser confocal microscope. CCL 149 cells were treated with different concentrations and for different time of recombinant protein FIZZ1. After treatment, the expression levels of interleukin 8 (IL-8) were detected by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>When CCL 149 cells were exposed to cigarette smoke extraction, FIZZ1 mRNA and protein levels expressed significantly higher than control group. Recombinant protein FIZZ1 promoted the expression of IL-8 in a dose and time dependent manner in a certain range.</p><p><b>CONCLUSIONS</b>Cigarette smoke extraction activates FIZZ1 at mRNA and protein levels in CCL 149 cells. Recombinant protein FIZZ1 induces the expression of IL-8 and may thus participate in the process of chronic obstructive pulmonary disease airway inflammation and airflow obstruction. Generally, immune cells such as macrophages, neutrophils and lymphocytes are unavoidably involved in airway inflammatory and immune responses to cigarette smoke, but it is still unclear whether their involvement in the pathogenesis of chronic obstructive pulmonary disease is based on the specific expression in lung epithelial cells of FIZZ1.</p>
Subject(s)
Animals , Rats , Cell Line , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Epithelial Cells , Metabolism , Interleukin-8 , Genetics , Metabolism , Nerve Growth Factor , Genetics , Metabolism , Pulmonary Disease, Chronic Obstructive , Genetics , RNA, Messenger , Genetics , SmokingABSTRACT
<p><b>BACKGROUND</b>Vascular endothelial growth factor (VEGF) can specially target the vascular endothelial cells so as to enhance their proliferation and angiogenesis. The aim of this study is to investigate the inhibition effects of VEGF antisense oligodeoxynucleotides (VEGF ASODN) on VEGF expression of Lewis lung carcinoma cells.</p><p><b>METHODS</b>The phosphorothioate-modified and liposome-encapsulated VEGF ASODN, VEGF SODN were added into the medium of Lewis lung carcinoma cells respectively. VEGF mRNA and protein were detected by RT-PCR and immunohistochemistry. And the proliferation of vascular endothelial cells treated with supernatants was determined.</p><p><b>RESULTS</b>VEGF ASODN could significantly inhibit the expression of VEGF mRNA and protein expression in Lewis lung carcinoma cells, as well as the proliferation of endothelial cells.</p><p><b>CONCLUSIONS</b>It is hopeful that VEGF ASODN may become a new antiangiogenesis therapy strategy in lung cancer.</p>
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Objective: To study the inhibitory effect of VEGF antisense oligonucleotides (ASODN) on growth of Lewis lung cancer in C57BL/6 mice.Methods: Lewis lung cancer cells were cultured and implanted subcutaneously into 40 C57BL/6 mice, which were then divided into 4 groups: VEGF-ASODN treatment group, VEGF-SODN treatment group, BEGF-MODN treatment,and control group (normal saline). Mice in different groups were treated 24 hours after cell inoculation. The weight and volume of subcutaneous tumors was measured and the morphological changes of tumor cells was observed under microscope. VEGF protein and microvessel density were examined by immunohistochemistry.Partial tumor tissues were kept in liguid nitrogon.Results: The average tumor weights of the control, VEGF-ASODN, VEGF-SODN and VEGF-MODN groups were (7.33?0.71)g, (4.56?0.38) g, (7.59?0.32) g, and (7.62?0.39) g, respectively. The inhibition rates of tumor growth in VEGF-ASODN, VEGF-SODN and VEGF-MODN group were 43.8%, 5.5% and 3.1%, respectively. VEGF-ASPODN obviously inhibited the tumor cell growth and decelerated the tumor cell proliferation. Immunohistochemistry results showed that the expression of VEGF in ASODN group was remarkly lower than those in SODN group, MODN group and control group (P